Invitro Induction of Shoot in Medicinally Important Plant Hybanthus Enneaspermus (Linn.)

Asian Resonance (P: ISSN No. 0976-8602 RNI No.UPENG/2012/42622 VOL.-6, ISSUE-3, July - 2017 E: ISSN No. 2349-9443)

Abstract


 Prashant K. Patankar

 Tissue Culture Laboratory, Deptt.of Botany, Shri Chhatrapati Shivaji College Omerga, Osmanabad Maharashtra, India.


Sanjay R. Biradar 

Assistant Professor, Deptt.of Botany, Shri Chhatrapati Shivaji.College, Omerga, Osmanabad, India.

            In the present investigation, Hybanthus enneaspermus is an medicinally important plant. It is known for its therapeutic value in folkfare medicine. H. enneaspermus shows the presence of alkaloids, flavonoids, carbohydrates, steroids, tannins, glycosides and terpenoids. Traditionally this plant is used as an tonic, diuretic, demulcent, aphrodisiac, in dysuria, sterility, diarrhea, and in urinary infection (Yoganarasimhan, 2ooo).In an attempt was to observe effect of different growth hormones viz., BAP (Benzyl amino purine), KIN (Kinetin) on invitro propogation in medicinally important Hybanthus enneaspermus plant. The shoot and nodal region of Hybanthus enneaspermus used as a explants for invitro culture and different combinations of cytokines in the Murashige and Skoog medium (Murashige and Skoog, 1962). Shoot formation in maximum number has been resulted in the MS medium supplemented with 9.0 µM BAP in which 14 shoots and average length of the shoot measured is about 14.9 cm. and MS medium supplemented with 10.0 µM KIN in which 12 shoots and average length of the shoot measured is about 6.9 cm. The initiation of shoot takes place in 25 days after inoculation and observations were measured after 45 days after inoculation.

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http://www.socialresearchfoundation.com/upoadreserchpapers/1/167/1907110735291st%20sanjay%20r.%20biradar.pdf

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