Asian Resonance ISSN No. 0976-8602 RNI No. UPENG/2012/42622 VOL.-10, ISSUE-2, April 2021
Paper Submission: 03/04/2021, Date of Acceptance: 15/04/2021, Date of Publication: 25/04/2021
Nihar Ranjan Singh
Assistant Professor,
Dept. of Botany,
Ravenshaw University,
Cuttack, Odisha, India
Abstract
Dyes are important molecules for coloring different
materials for better visualization. Synthetic dyes are harmful, nonecofriendly and toxic to human health as well as surrounding
environment. Synthetic dyes are usually discarded into the
aquatic system and potentially expose humans and local biota to
adverse effects. Therefore, to substitute the harmful effect of
synthetic dyes, natural dyes are a better choice. Natural dyes are
ecofriendly, non-toxic, non-allergic, non-mutagenic and cheaper
in cost. Proteins in SDS-PAGE gels are stained with chemical
dyes like coomassie blue, silver stain, zinc stain and fluorescent
dye. These dyes are expensive and hazardous to health. All
these synthetic dyes used to stain proteins in SDS-PAGE are
more or less self-reactive, flammable, having mild to acute toxicity
(oral, dermal, inhalation) and skin irritant properties. Alternatively,
natural dyes can be a substitute, which is easily available and
their making procedure is much cheaper in comparison to
synthetic dyes. Protein sample stained with selected natural dyes
were observed on SDS-PAGE and evaluated for being their
effectiveness in binding protein. Further, isolation of natural dyes
and their staining compounds can be considered an alternative to
synthetic dyes in protein staining process
for
full paper please visit below link :
http://www.socialresearchfoundation.com/upoadreserchpapers/1/420/2105270612361st%20nihar%20ranjan%20singh%2014067.pdf
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